Improvement and breeding
H. Mohammadi; M.R. Pirmoradi; M. Moghaddam; M.H. Shamshiri; M.A. Mirzaabolghasemi
Abstract
Basil (Ocimum basilicum L.) is one of the important plants from Lamiaceae family which is used as a medicinal spicy plant and also as a fresh vegetable. Salicylic acid (SA) is one of the most important plant growth regulators that regulates growth and physiological development and metabolic processes ...
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Basil (Ocimum basilicum L.) is one of the important plants from Lamiaceae family which is used as a medicinal spicy plant and also as a fresh vegetable. Salicylic acid (SA) is one of the most important plant growth regulators that regulates growth and physiological development and metabolic processes of the plant. Its application under salinity stress conditions decreases the effects of salinity toxicity and makes resistance in the plants. To investigate the basil (O. basilicum cv. Keshkeny luvelou) physiological and biochemical responses to salinity stress and SA, a factorial pot experiment was conducted based on a completely randomized design with three replications in the research greenhouse of Vali-e-Asr University of Rafsanjan. Experimental treatments included salinity at four levels (0, 30 ,60, and 90 mM) and SA at five levels (0, 0.5, 1, 1.5, and 2 mM). The results showed that salinity stress reduced the amount of chlorophyll florescence, chlorophylls a and b, total chlorophyll, carotenoid, relative water content, and essential oil percentage. The SA treatment decreased the negative effects of salinity stress on the plant and improved essential oil percentage. Also, salinity stress increased the amount of proline, soluble carbohydrate, phenol, antioxidant activity, electrolyte leakage, leaf and root sodium, and chlorine, but the SA treatment at some salinity stress levels caused to decrease and/or increase these traits. Overall, SA improved the physiological and biochemical characteristics of basil under salinity stress through increasing osmolytes production and resulted in increasing nutrient uptake and essential oil percentage of the plant.
M. Shokrpour; S.A. Mohammadi; M. Moghaddam; S.A. Ziai; A. Javanshir
Volume 24, Issue 3 , November 2008, , Pages 278-292
Abstract
To investigate the relationships between morphological, phytochemical and molecular markers in milk thistle, a set of 32 ecotypes collected from Iran along with two introduced varieties, Budakalaszi and CN seeds, were evaluated. Canonical correlation analysis between 8 morphological attributes and 7 ...
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To investigate the relationships between morphological, phytochemical and molecular markers in milk thistle, a set of 32 ecotypes collected from Iran along with two introduced varieties, Budakalaszi and CN seeds, were evaluated. Canonical correlation analysis between 8 morphological attributes and 7 flavonolignan compounds forming silymarin revealed that first two canonical variables showed high canonical correlations. The loadings of the canonical correlations indicated that ecotypes having higher values for 1000 seed weight and lower values for flowering time, plant height, capsule diameter and seed yield would have higher silychristin and silybin and lower silydianin contents. In other words, larger seeds would have higher silybin and lower silydianin. Out of 415 polymorphic markers, 37 and 29 markers showed significant association with flavonolignans markers and morphological attributes, respectively. The informative markers showed 54 and 45% of the variation for taxifolin and silychristin, respectively. In the case of morphological traits, more than 40% of 1000 seed weight, flowering date and plant height variation were determined by informative AFLP markers. Results of the study clarified that some of qualitative and quantitative properties of essential oil in milk thistle can be well predicted by morphological and also molecular markers.